A Novel RP-HPLC Method for Simultaneous Estimation of Metformin Hydrochloride & Glimepiride in Tablet Dosage Form

A new simple, fast accurate and reproducible reverse phase high performance liquid chromatographic method has been developed and validated for simultaneous estimation of Metformin Hydrochloride and Glimepiride from tablet dosage form. The method was developed using Waters HPLC system on C18 column (Spherisorb ODS 2: 250mm x 4.6 μm) using a mixture of 25mM Phosphoric Acid pH 3.0 (with KOH) and Acetonitrile (40:60 v/v) as mobile phase in an isocratic elution mode at a flow rate of 1.00 ml/min at 40oC with a load of 20μl. The detection was carried out using UV-Visible detector set at 240 nm. The retention time of Metformin Hydrochloride and Glimepiride were found to be 3.20 min and 6.7 min respectively. The method was validated with respect to linearity, robustness, precision and accuracy. The method had been successfully applied in other pharmaceutical formulations of the same composition.